University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

60

Pages:

2
Author:

Sanjeev K

28EXPERIMENT No: 12DATE 29/11/22ABO BLOOD GROUPINGAIMDetermination of Human Blood groups (ABO) using MonoclonalAggutinating antibodies (Antisera).MATERIALS REQUIREDGlass slides test tubes, disposable applicator sticks, normalsaline paster pipettes centridye disposable gloves,calcium hypochedic solution (5%) to wipe if disinjectthe Na spells a / disposable waste bay to collect 4 dispore usedaccessories of wastePROCEDURERapid slide test1perpace 10% suspension of RBCS in normal saline Alternativelyoxalated whole blood can be usedMack 3 circles on a glass slide al label then as A,B, EA2.3.Place 1deop of the appropriate blood grouping anti-see atthe above marked area4.Place 1deop of 10% all suspension or oxalated whole bloodto each of marked allaMix well with reperate applicator sticks 4 spread it over5.an alla of 2 2 mm diameter , lilt the slide back oftorth for 2 mins.observe macoscopically for the evidence of aggletination.6.OBSERVATIONNo ayfutination was found when blood mixed wasin with antiselum A / B 4 D. 29COULTshe blood group is o-ve. ohn no antigens.but both anti-A of na antibodies in the plasma athell Rh is negative.DISCUSSIONABO blood rap system, the classification of hemm bloodneed on the inhecited properties of RBCs as determined bythe presence or absence of the artigers A al B which allcarried as the surface of the red cells. Persons may thushave type A, B, O a AB blood. The A,B, 40 bloodgroups well 1st identified by Austrian immunologist Karllandstaines in 1901