Urease Test Textbook Notes - Edubirdie

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

211

Pages:

2
Author:

Sanjeev K

19VIUREASE TESTAIMTo check the ability of the bactura to produce weaensymePROCEDUREveea is added to the autoloved usea agar base as per1.the protocol written in the Hi media bottle2.Fresh inoulums from an 18-24 hr pull culture wasinoculated into wease broth al incubated withloosened lap at 37°C for 12 - 24 hes.3A colour change from yellow to pink was taken as apositive reaction.OBSERVATIONA pink colour was observed at the top surfaceof the slantRESULTThe given organism is veease +ve.DISCUSSIONUkease last is a biochemical test that detects the alkalinefermentation of usea with the resultant production of NH3by microoyanisms. The termentation of urea occurs in thepresence of enzyme wease resulting in 2 molecules of NH3al CO2 urease activity is one of the important characterishiestoe identification of Proteus to be discinguished feam non-lactose termenting members of the Enter bacteriacualDuring the test , the organisms utilise wea assole source of nitrogen, producing sufficient amount 9NH3 to overcome the buffering capacity g medium Thechange in colour of medium as a result of the change inRph is indicative of the test result cotton plugTest tubePink colour(+)controlusC

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