University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

381

Pages:

2
Author:

Sanjeev K

3EXPERIMENT NO:2DATE - 11/10/22ISOLATION OF BACTERIA FROMWATER AND AIR SAMPLEAIMto whats Instruction fean environmental sampleMATERIALS REQUIRED1 Nutrient agae2. Steele swates3 Felli plates4. Test tubesPROCEDURE FOR ISOLATION OF BACTERIA FROM AIR1)150 ml of nutrient agne was prepared as placed in theauto lave along with are petrip lates for stecilization2)After stecilization ,the nutrient agar was pareed in thepetriplates al allowed it to solidity3)After the plates all solidified, all the plates all taken airall the laminar air flow of kept in the openfor different of internals of lime ie, 5min (s splates) 10min (2plates) El 15 min (2 plates).4)/ plate is kept sepalately inside Laminae All How for5)linted Ague exposure of plates to air , it is incubated at 37°C for24 hours6)After 24 houes the bacterial wonces were observed.OBSERVATIONThe bacterial colonies were formed at different time internaland different merphology of colonies well obseeved.RESULT12 whonics well formed when the plates well exposed for5 min , 24 colonics well formed when the plates well exposedfor 10min ,30 colonies well formed on the plates whenexposed for IS mins. 4PROCEDURE FOR ISOLATION OF BACTERIA FROM WATER1150 ml of nutritional agai was prepared2.7 tubes containing 9ml of each dissilled water was prepared3Test tubes peticiplates up nutrement agal was kept for steriligationusing autoclave4type stecilization add Ind of water sample to the test tubecontaining Iml of distilled water (This is 10 -1 dilution)5.FROM each dilution 1ml was taken as made upto 10 dilution6Nutrient agar was powed in the petriplates 4 allowed itto solidity?After serial dilution af solidification of nutrient agal1ml of sample was pepetted into the petriplatesThe 0 entire surface was carefully spreaded using steele L shaped8glass rod.9.The plates well incubated at 37°C 101 24 haves.10.After 24 have of incubation the bacterial colonies wereobserved al calculatedRESULTSBacterial donies with different characteristics werefound to be grown in both pend and tap waterDISCUSSIONUnlike soil 4 water ail does not contain nutrient materialto support growth of micro organisms it acts as medium for transferof mirobial propagules Microbes in air generally come fromsal water al living organisms activities. The micropora of airis highly dynamic af is affected by timp, wind, humidity 4other human El animal activitieslife on earth It may become the place for the growth ofwater form a major source is supporting all forms differetypes of of microoyanisms which may have a potential for spreadinga variety of diseases. The mal the amount of bacteria in waterthe more it is to drinkRegular isolation of bioarcesds 4 water nicobeshelps to understand the quality of air 4 water.