University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

205

Pages:

2
Author:

Sanjeev K

Date 26/05/235Expt. No.Page No 10ISOLATION OF XENOBIONT DEGRADING BACTERIA BYSELECTIVE ENRICHMENT TECHNIQUESAIMTo isdate renobient degeading bacteria by selective anrichmenttechniquesPRINCIPLEBiodegradation of petroleum hydrocarbons is a complex processthat depends on the nature Cy on the amount of hydrocarbonspresent Peledeum hydeocarbons can be divided into 4 classes: the saturates the aromatics the asphaltenes 41 the retins.The susceptibility of hydeocarbons to microbial degradationcan be generally ranked as follows: linear alkanes branchedalkanes small alomatic cyclic alkanes the extensivebiodegradation of alkyl aronatics in marine sediments whichoursed peior to detectable biodegradation of n. alkare peopleof rude oil 4 the micooganisms namely ArthrobacterBuskhadderia Mycobacterium Pseudamonas, sphingomonas4 Rhodocouns were found to be invelved for alkylaromaticdegradationMATERIALS REQUIREDEnrichment beath soil sample , arposed to oil, petriplatesconical flask.PROCEDUREEnrichment of oil degrading bacteria:-/the soil sample exposed to oil was collected ujused forTeacher's Signature DateExpt. No.Page No. //isdation of oil degeading bactelia2Enrichment loan was prepared by autoclaving the media31g of soilsample was weighed 4 added to the enrichment brothaseptically alincubated in the increator to , day at 120 rpmIsolation of oil degeading bacceria :1.the enrichment broth showing turbid bacterial growthwere further speead plated on to enrichment agai whichwill selectively isolate the oil degrading bacteria2.The plates were incurated at 37°C for 5 days.3the alony growth was observed cy maphologywas recorded.OBSERVATIONAltre performing gean staining pink coloured rod shapedbacteria were observed under 100x oil immersion microrope.RESULTThe isolated xenobiont degeading bacteria is geannegative bacillusTeacher's Signature