University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

483

Pages:

2
Author:

Sanjeev K

EXPERIMENI NO G DATE: 24 8 2022 NMR SPECTROSCOPY - Demeretration He is а притопоре сechnique whain is was to duermsure the mucore of unemical compounds The twem Nime can be defined as 'N' propertica of atomic nucler, 'n' o 'm imagnetic field field is meum asy and 'R' It is the interaction Poetuselin rodio frequency and magnetic field. Here, in Nue spedro- mopy hubstances are moguns is igh frequency nadic waves (umally 300-500 MH₂) oling "custo large magnetic field. In the presence of the nuclear ma magnel an external magnetre field vitner aligns to tsarde de magnetic field. coined the magnetic So it Mars; a-notation (2) and susults in woepin B-srotation(-12) Opun flip occurs when muler moves from ground state (lower energy) to excited state (higher energy) by the absorption of Energy In a simple manner the instrumentation of NHR unwists of; supermooded magnes RF Recorder and Detector. Reusable NMR tubes are used for loading the sample. The NMR comists of time cylinders; the first one 15 the super cooled magnet around the sample loaded Nime tubes whion is surrounded by liquid helium (-269°C) which in tum is surrounded by liquid Nitrogen (-1960). Here He is used to keep the magnets wol and liquid nitrogen is used to prevent the evaporation of He and also to absorb heat from the magnet Aldosampton oru und where many samples ara loaded by one simple may be analysed at a Live Rou to NMR deuterated solvente men as Acetone and cuts are wed Tetrametnyt silane (TMS) is used as reference sample. NHR wmok receives and transmith RT. It has got an input and output. The operator gives message to the wnsole which are Passed to the magnet and then it is send to the console for reviving high is finally passed on to the frequency radiation, which work station, le computer. to obtain the NHR Spatrum. However, in order to analyse emy unknown compound NMR alone is suffi went, but all other techniques of laboratory be used such as not Sophisticated GIC-MS, UV, Visible, IR etc should