University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

483

Pages:

2
Author:

Sanjeev K

EXPERIMEN No.6nDATE:24112022NMR SPECTROSCOPY-DemonstrationNMR is a sputrotropic technique which is uses todetermine the invicture of unemical compoundsThe turn NMR can be defined asi'N'- propertiesof atomic nueler, 'is' a magnetic field is newsaryand 'R'. 11 is the interaction betwein radiohapy are imported to high frequencyfrequency and magnetic field. Here, in NMR spectro-radio waves (unually 300-500 MH2)aling withlarge magnetic field.In the presence of an external magnetic fieldthe nulear magnet either aligns towards in oragainst the magnetic field so It results woopinstates; a-rotation (+1/2) and p-retation(-16).gounflip occurs when nulls moves from ground state(lower energy to excited state (higher energy by theabsorption of energyIn a simple manner the instrumentationof NMR convists of ; superioded magnetsRF transmitterRecorder andDetectorRelisable NMR tubes are used for loading the sampleThe NMR convists of truee cylinders; the first oneis the supercooled magnet around the sample loadedNMR tubes which is surrounded by liquid helium(-269°C) which in turn is surrounded by liquidNitrogen (-196°). Here 'He' is used to keep the magnetscool and liquid nitrogen is used to prevent theevaporation of He and also to absorb heat from the 12magnetAcoto samplers are used where many samples atare loaded, bell one sample may be analyse a as atime Hou in NMR deuterated solvence suchacctone and CHCL are used Tetrametryl silane (TMS)is used as a reference RT. 11 has sample got an NMR input console and receives outputThe and operator transmits message to the console send which to the areconsole to the receiving high fre quency station radiation, ,ie computer, whichpassed gives magnet and then It isis finally for passed NMR on to spectrum. the work However, in order alone is to notanalyse any but all other techniques IR etc shouldto obtain the unknown compound NMR of sophisticatedsufficient laboratory such as GC-MS, UV, visible,be usedgotfrom