University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

193

Pages:

2
Author:

Sanjeev K

0016219-12-2022 LIGATION PROCEDURE I plau Ta DNA Ligare at a 15 my on the pipette 141 of ligase to the viat 2. To the nal labelled as ligated sample add 10,ul of NECOR, digese Add 10ml of 2x ligase assay. buffer mix by tapping 3 4- Incuball at 16°C for Test-14 DNA 2brs in pre set water bath. Ligase + X lEcoR, digest + 2x assay buffer 5. Prepire 1% agarose gel 6. Add aul get loading buffer to ligate a sample at the end of 2 hrs. 7. Pipette our 10ml /EWR, digest, aul gel loading buffer and 10-12,41 water (control) in to vial labelle of as Control 8. 20ad the ligated sample and control sample in the walls gis agarose gel 9. Elutrophoress the samples at 50-100V. Visualke in uv-trans, Iluminator. OBSERVATION Orange colowe d bands were Observed, that is in control A bande were obsomed ul in test sample I band was obsemed, which indicates that the DNA sampki ligated. RESULT The following DNA sample was ligated