University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

497

Pages:

2
Author:

Amal J.

Dye Reduction Methodthe included on other redux potential. The redox potentialorganism obtain energy be chemical reactions involving eitherorganic / Inorganic Compounds This involves an oxdn . redesreaction ie, the source is oxidized and the other com pound isreduced The rean are concerned with e transfer.when a compound loses an e it is oxidized. So compounds varyin oxdn-redn potein potential (ie, redox potential)So being determined potentiometrically H can be measured usingIndicators / dy.Color charge x metabolic vate of CultureSo ,larger the no: of cells 1 sooner the colour changeMethylene blue (dye)Resazuris ( Indicate) (blue)due to the lose of looselybound oxygen to to atonredn.Resoryn (pink)leuco- methylene blue.revarible by atmosphere O2neetherDihydroverofin (levio)bdneg. used to determine bauterial quality of milk and dairy pdts.Such as increaseRevazumDetermine Quality of fresh Scallop meat Tripherystabazolium chloride (leus)Furmazan (red) bacteral activity.-This is used to distinguish bacterial colonies from01food particles in an SPC.BraduaAdvantages of Dye redn testEstimate baterial contamination in a shorter timeMethylen blue and tehazolium can be used asInhibitor too ..DisadvantageReducing enzymes in good can cause colour changeso, the test indicate more contamination than alreadypresentMost Probable NumberStatistical method used to estimate the Viable noof bautena in a sample by inoulating broth in10 fold dilutions.