University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

187

Pages:

2
Author:

Amal J.

BiosensorsIUPAC define biosensos as a doure that uses specifebiatherial reactions mediated by Isolated enzymes, immuntissue organelles, or whole cells to detect chemical compausually by electrical thermal or optical signals'Analyic devile that has two main elements:a) Bioreceptor .b) transducerBioreceptorBiomolecule that rewgnizes the target analyTransducerConverts the recognition event into measuralgrower standing briSignal.Analytical device which utilize biologial reaction ofMbiahemical molecule for detecting target analyte it and corvea biologial response into quantifiable and ProcessablesignalBioreceptor can either bea) Biological material- - Enzymes / antibodies, nucleic acidand cell receptors.20015 Biologically derived material Aptamers (single strands degreesthat fold into defined architatures and birdtargets Proteins). recombinent antibodiesCBiominic - Imprinted Polyners and Synthetic Catalyst.Transducer can bea) Opticalb)Electro chemical2Mass - basedof Thermometric .2) Micromechanical / Magnetic.Biosenson easy to operate because they donot requirepre enrichment, unlike nucleic acid to and Immunologicalmethods (they require 80 pre-enrichment for concentratingthe pathogens before detection).Recent bioseneor used to detect food-burn - pathogens areOptical electrochemical and mass- baied.OpticalIMost commonly used optical biosensor is (food burn pathogen detection)is Surfaie plasmon resonance (SPR) biosensor due to itsSensitivity.J.INEmploys Reflectance Spectroscopy for Pathogen detectionIn SPR biorcepton are Immobilized on the surface of thin metal