University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

141

Pages:

3
Author:

Amal J.

Genetically modified LAB for Various Application (Sirstille)LAB have. a long history of use. in formented food products.-Progress in gone technology allows their modification by introducingnew genes or by modifying their metabolic functions.There modifications may lead to improvements in food technology(bacteria better fitted to technological processes leading to improvedorganoleptic properties) or to new applications including bacteriaproducing therapeutic. molecules that could be delivered by mouth,benetically modified LAB can produce desired compounds as cell factoriein closed systems, which reduces the possibility of the unwanted releaseof microorganisme into the environment and the risk of contaminationsuch applications are therefore a lot. less. problematic from the regulatorpoint of view.IGenetic engineering of LAB has enabled their application to theexpression of recombinant proteins.the most common genera. used here are Lactococcus andLactobauillusJMetabolic the engineering of LAB allows modified modification eyexisting metabolic pathways to improve the properties of LAB as food fermentation startersPerfore metabolic engineering strategies have been used torevoute metabolic. pathways for the production of sweeteners,flavors / aromas exopoly Saccharida and vitaminstoreover through metabolic modification GM- LAB canproduce metabolike with health benefits or high valuebiochemicals, with better production yields achievedLactic AcidMain industrial application of LAB is in the formentativeproduction of lartic acid / which is also the primary productof their Carbohydrate metabolismtoLautic and has a long history of use in food rosmetics,pharmaceuticals, chemicals and agricultureMoreover the demand for laute and is growing on accountof its use as a precursor for production of biodegradab leand biocompattible polyantic acid, with has commercial valuein the fiber, textile, plasticulture, and packaging industries.Genome shuffling was applied in Lb. tham nosus to improveglucose tolerance and at the Same time to chhanceL-tactic acid pdn -Disruption of D-loutate dehydrogenare ( 1 dhD) gene. 1 L- lactatedahydrogenare gene ( 1dhl) resulted in the formation of opticallypure. L and D - lautic acids, respectively by Pedio coccue acidilacticiand D- lactic acid by Lb. plantarum12h D gene deficient Lb. paracarei and Lb. helvetime have beenused to produce L. lactic acid.TDepardent on the species of lautobacillus production of L- D-or LD-lateand is possible.The intrinsic production of compounds of interest is preferable butthis can often result in insufficient quantities. Genetic modificationoffers the possibility to increase the yield of the compound of interest,as well as to enhance the characteratics of the compoundleg its purity).to increase the pdn of optically pure lautic and gnome. shefflingand disruption or deletion of the lactate dehydrogenare (1dh)gene has already been applied.As well as the production of lactic and LAB have roles inindustrial production of non - -food products 1 eg: ethanol pds)and functional ingredients , Such as vitamins ,low caloneSweetner exopolysaccharides and antimicrobial agents.