University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

216

Pages:

4
Author:

Amal J.

Electro phoresis :-1Things can varieyVoltageAgaroseGel matus.buffers / saltsprobwhile struckingAlways load marker.Malrix , samples according to this, different types. / /:Denature the samplesSDS pagedeligentSo that it can move easily.B mercapto ethonolDNAdenature ?Continuous and discontinuous buffer.Agaiose buffersame buffercontinuous.polyacrylamidebuffer system chargesdiscontinuous.when pH charges, change of protein also changes.Different types(Mostly qualitative)1]Gel electrophoresismatrix. (quantitative can be2]capilaryclone.Separation - Molecular weightTypes of RNA.catalylic activity of a protainZymographycheck.protein ag usedfor in electrophenesis.hour md wb = Cytosine.Get more onfo from mol interest.Hemoglobin4 discuplide bonds. .white running PAGE - 2 banes. Rune bitonentargle bcoz length. of/ /Globular protein & Fibrous proteinfactorsDyebromophand bluegrotaffectingloading dye - compositionrelycharged above the pHSampletrely charged below the pH.Electic field.MediumAfter GelNass analysisBuffer.To get a complete infoProtein Stacking of resolving gel.2% gel decliophoreic of DNA-X=what you can change for running you sampleBalance not by tube by weightFibrous proteinendanglelater thanClobular proteinAt PI they will not move.Any common protein which may not run properlycharge based ProteinAHistonesFactors affecting electrophoresisSample , Sike , charge shape. /_/electric fieldMobility of the ionin potential gradientScs the rate of migrationMediumNot same buffer.Otheus cue also chargedcharge.Depending on the pka of Alyune, the change willAllaa have 2 pkaMeans it have 2 acidic groups.one place chargedPInet change is hero." not chargedAps 9 TEMED.