University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

107

Pages:

5
Author:

Amal J.

Gas chromolographyNot using that muchSamples gaseous.has to be voladele.GSC - Solid (adsorption)GLC - Liquid (pacition).use temperalure to control the volatle sampleThe more volatile comes first and it is hydrophobicEarlierfathyacida analysis aredoneto / indmol characterization.NowIGSrDNAFAMEFatey acid methyl ester.Run in GC.Creams biochemical FAMELonger column bettu repdation.it absor bs higher furquency emits at lowel frequencyin visible range.Pro env H20 1/10/23Many probesGreen fluoresence proteinBiggerEthidium bronideintercalating agentLipids which are volableGC.materials range from polymers tomolten salts of liquid crystalsopentulular ColumnPacked columnwall coated or liquid CoatielDetectors:-1Thermal conductivity detector.Get backer sampo2Flame iorization detector3Nillogen phosphorus detect or.4Election capture delector.5Has of personalityAdu & advert / /Rapid Scanning FTIR detectorMars Meclonetry deletorCH3CH2COOM ambhipaticCM3 CH2(M3 hydoophylicRetention -The time required to dute the sampleHigh resolution" Speel" SensitivelyI AwayMelhyl parathon 9 - PericidesMalalitionorganicEtionHighestFirstLowesLustprace functional & of n/g 3.nMRNA HMusMRpol Aulusmy GF Numn .Aven & 150Queswhen / ask you to characturize a flawrenceprotein / material aProblem you face in therescanteSome material absorbMulagenesis (Random / Site directed)H PLC :-performance.FPLCFast flow Liquid chromatographyion exchange pressure is not high as HPLC.we are UU detectors in FPLC.Protein mobile phasebuffer.2 avoid non specific binding.High perfumance wg/ chanalography.microporesultia HPLC (column is smalle Runs can befaster. Hight equivalent to a theritical plate/ /HEPT HETP = HNLargersmallerN= 16 (tr I wb)2 orN= 5.55 ( tr /wi/2)2TRRetention timewbwidth of the base.Selectivity and capacity factor.K'A=MtR - tMin GCTemp changed.tMHPLCgradientAbsentSaturdayReverse phaseautonituile