Gell Filtration Chromatography | Swansea University - Edubirdie

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

443

Pages:

5
Author:

Amal J.

Gul filluation chromatographypermeationMolecular Sieves.why you are using ammonium ?Con exchangekeep on exchanging buffer -HPLeNormal phaseReverse phaseFermentedTLC I Paperlosteven nowGlutanic acid E cipalic acid.CH3hydrophrice.CH2CH2 ProteenCH2 G12- velyPartition coefficient / /Affinityalywprocai orcloser to electronegarivedownfield ofSputting of H is due to neighboringatonsno HHS of 2 different environmentQuerion:2g/l Soln. Transmits 75% How much 4g/ltrasmits?E=glrdT= 75%A=8clA= + log TT= It01 T T = ( It) X100101075 = ItI- log T = AT= 1/10- log 75 = AT=11T=75 = 0.75100 / /A= -log T= - - BogorsA= 0.124A= Ecl0.124 = Ex2x1E- 0.062 .A=Ecl= 0.062 X 4x I= 0.248 .A = 2-logT0.25 = 2-logTA = -logiartilg (0.248) =Quesa = 7400 A° findwave no & frequencyC= avV= CA=10 unitsaV= 2.99 x188mls740 $ X 10-90= 9140 4.05 X10 / /wave no = \a=\o =\= 17400A 740nm 740x105= 1.35x10 cm0.3 atQuestionNADG NADH minutes OD at 340 (6220)1.2 at 260E =1800isoon>nNADHOD at 340.1.2 at 260 2 15000.3 at 340 - 6220Find how much NAD Ey NADH are there?NATA FNADH at 340 =0.30DA= Ecl0.3 = 6220 x e x 1B= 4.8x105C= 4.8 x105NAD FNADH -A=Ecl.A =Ecl.18.1.215xx1C =A = 75 / /A=Ecl2 - 1500 r cx11.2 = C150005.8 = C= 15000 x4.8x1= 0.721.2-0.72=0.45A= E10.45 = 18000xCX10.45 = C180002.5 8155 = C.EuviumC = 2.2x10 -5. MolarFind TransmittanceE = 62201.5000A= Ecl= 15000 X 2-2x10 x 1= 0.33a artilog of 0.33Transdace= 2.131=2.1372183IT= to =2.137= 0.46

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Gell Filtration Chromatography

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