University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

418

Pages:

4
Author:

Amal J.

MicroscopyMagnificationResolution.closely Spaudclear visualization2 particles diffractbecomes blurXMagnification.2 things should be seen as different.Combination of lenses to get a magnification.ResolutionLightwhen I it changes from onemedium to anotherspeed changeSnells law- nisin our I n2sino / /o = anglen1 = RI of 1st mealiumn2 - R2 of 2nd "muchNumerical aperture = It tells how lightCan gather It is an in desn Sir O.Q = 1/2 angle of the cone&9cone ofcapacity of gather light ismagnificationlightobjective lensmagnification.Eye lens (piece)should include photograph bragnifiedfFocal lengthimage will be enlarge andlaverted .inverted virtual image.2Resolution:-If should be smallRMagnificationDepends on the wavelergth of the light usedand NA.Light =RatoNANAwavelength should besmall. 331/2/ /keep changing the light CUV, IR, EMR).2a should be smaller.UV should be used.e- can also be used. So it a is Smallerthan light. Hence elections can also be used.e should be used in vacuumType and purpose.oil refractive cindex is higher.angle should be more to gather lightfor air = n=1for oil or glass n= 1. SIResolution = ar=a / 2NA .r&aNAbetter resolution LightUV = small.inverted microsopecells are adherent. To see the Sample upside down.Compound miuosopeBright 4 dark field micoscope.(adeLook atabsorition (scatling)unstand sample)high sourcimercury lamps , xenon lamps , Le LED.phase contrustupliftInstrument itselfphase contecut dark.light/ /distruction & construction.phase contrastConticest becomesMin between stages.dark fieldannular diaphragmSelectively focus the cellsIn phase contiantplatediffacted go all sidesnot distractedwill come out.Principle of fluorene miuoscopepreparation of Sample is toughpass one type of light and absorbs and emilsout in crothes light.Hence 2 types of filler are used.absorb lower Wavelergth umits higher wavelergth.Tagged Ab and birds to itBetter monocheomatur -Naro filters. NaiowaveleggExitiation filterDicheomatic - Barrioemision filles.