University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

87

Pages:

5
Author:

Amal J.

Radio isolopes=1.How do you measure radioactivity?X ray film - one type of analysisGeiger muller Counter.Isolopes - same atomic number different marsnumber.Different no. of neutrons.Penclualingdvery lowconsidered saferrhigh emithingBmediumMix 2 (primary cy Secondary fluex)Units:Dpm = dN / at = anT1 2 - 0.6931aLanda - decay Constant2.303 log No / Nt = It 231g (MW) = 6.023 X10 atom/ /Quesk40 - 0.012%Human body - 0.35%CalculateRadioadivity resulting from 1x40 decay in75kg human.Total k40 = 0.012 X 0.35 X 75x103g= 3.15 x102 g.No. of atoms.23= 3.15x1529 20 X6.023 X10 atm 1g / rog= - 4.74 X10 atoms.atomyears)2 = 0.6931 X 365 x24x601.3x109- 1.014 X 1015 min-1-DPM = an= 1.014x10-15 min x 4.74x10 20= 4.818105 DPMConvert to miles make wrie DPS= 3.7 10calculateQues :- Ca45 - hay we 163 days.T1/2 = 0.6931 * 365 x24x 60.x63163 = 0.6931a / /Hay life , Decay .To count the H.tonwert to curilDPS and DPMBequirel (Bq) - onedpsone will = 3.7x1010 Bq,decay constant ?Ca 45 - Da 163 daysLTya = 0.6931a163 = 0.6931aa = 0.6931=4.25x103163=0.0042524x 60x60= 4.91. -2.303 log No = atNt2.303 log No I x904.25x103Nt / /2.303 log No = 382.5x1 103Nt 2.30321803 log( 100) = 1.6587xNO = No of atoms21=65/87t2x1.65871.2=1xlog 100 - log x - 166.08= 1.66x1052 - log IC = 1.66810 -52+2-1-66x155 = logx0.34antilog of 0.34 = ICQues:C14 =5700 yr.3fraction of atum decay per year2=0.693 = - 0.693 - 0.0001216T1/25700 = 00 1.216 xistTo make it into a = 1.216x10 1-4a whole number I . 8.225x103L>12164104/ out of 8.225x103 atoms decay per year. The base/ /1419Base value of 9 adjacent peaksm2m3msM51420, m2 +1310,1220, 1140, 1130H, - I = 1420 - -1M2 - M, = 1310-1420Me - M11310n2 = M2 - M,1419 -1419=Qwhy proteins are very sequence based?14191310O109Secondary structure30Pydrophobic interactions18001,21/CDcircular dichioism1140 1299absorption difference1220 1420130020 Sty acture120