University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

463

Pages:

2
Author:

Amal J.

Atomizationconverting free analyte to gas free atomRequires Solvert removal 9 volatization of analyteCuC12 desolvatio Cucl2 Thermalenergy2 cu (g) + 2cl (g)(aq)stram ofsamplenebulizer by passing a Righ pressure compreisedair passed past 'at the end of capillary tube in SampleThe Sample strikes the glass impact bead convering it intoa fine auosal mist within the spray chamber)by combustion gases Cais as acet yrone ) flames themalenergy desolvater the auosol must - arosol / fine paulesGraphite furnace over Flame.much more efficientcar directly absorb very small obsolute quartities of samplesprovides reducing environment for easiy oxidited elements.Samples are duesty kept in graphite funace G the funace iselectrically heated in Several steps to day the sample, ahorganic matter and vaperite the analyte atoms.photo diodecharge injection Devicechange coupled Device Intraction b/w a molecule cy IR radiation.Photon energies associated with IR are notlargelyenough to excite election but may includevibrationalexcitation of covalently bonded atoms E groups.2 regionsFunctional Group - 4000 - 1500cm - \Tingu print - 1500 - 500cmNO 2 molecular Structure have same IR spectiaIR - finger print of a molecule.virtually all organic gro compounds absorbIR radiation in corresponding with these energy.that corresponds in onergy with these vibrationsAn IR Spectrum shows that the energy absorbtionas one Scans the IR region of EM Spectrumwavelergth is measured in wave Numbers.V =I ACO 4aMost infofunctional group.The polar COV laint bonds are IR active henceexcitation car be seen in IR spectiumIn organic compds there polar covalentbondsrepresere functional group.Here what FG are present inside the molecule