Detector, Polymer and Plate theory | Swansea University - Edubirdie

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

205

Pages:

3
Author:

Amal J.

Detectors:-variable wavelergthNMRScarring 11Reflactive indexFluorescencelight ScallingElectrocremicalMass spectrometuspolymerDextran linked to closs lisked agaiose SuperdexK polyvinyl chlorideFractogelIn column chromatography, atheortical plate may be inagred as avery thin section which molecules can partition b/w mp and sp.withinThe thinnes the section, the better the performance.plate theorydeparation separate equilibriums of the Sample blue spand MP occur in these platesColumnTheoretical plate. A way of measuring Column efficiency either byHartingtheno of theoritical plates in a column, N.C the moreplates thebettu) or by stating the plate height , the heightequivalutto a theoritical plate C the smaller the bettu).HETP = LINL = Length of columnPlate Theory :-The plate height or height equivalent theoritical plates , H isthe length of the cdumn divided by no. of theritical plates.N = 16 (tr 1wb)2orN=5.55(tR/)2wi/2N = No. of platesbR = Retertion timetr

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