Non Denaturing Samples

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

98

Pages:

2
Author:

Amal J.

NON DENA TURINGSDS orUsed to protein in nature State without any treatmentNature gel ( polyacylanida, Starch I againse) are usedas these doesn't purposely derative the proteinThe native charge on the protein (divided by itsmass) detumines how last the protest travel and inwhat directionPro - tuely / -vely charged in soin dependingon their isoelectric points and the PH of the Solution.Pro - vely charged if PH is above Plil - tuely."if PH is below P1Various format :-one format is to apply small amounts of a sample toa support (gel) and follow allow the analyte the samplesto bravel in a running buffer through a supportwhen an e field is applied. ( Ceal dedrophonesis)It is also possible to Separate the components ofa Sample by using a narrow capillary that isfilled with a running buffer and placed in ane field 1 capillary electrophoresis) Factors affectingSample / Electric field / medium Buffer.Sample:-change / mass ratio of the sample detects its electrophonetic mobilityThe mas consists of not only the size and also the shapeof the molecule.charge :-charge electsophoretic mobility change is dependenton the PH of the mediumSixe :-molecules (bigger) Small m electrophoretic mobilityCompared to Smaller particlesShape:-Globular pro migrate feuter than the fibrousproteinElectric field:-Rate of migration undu unit polential gradent isreferred to as Hobility of the ion.An in polerti al gradentthe rate ofmigrationMediumcan exect adsorption Cy molecular Sieving effects on theparticle in luving the rate of migration.Adsorption :- Means relection of a component on the SurfaceRate Cy resolution of electrophonetic Separation can beof supportery line mediumreduced by addorption.