Recommended Stationary Phase

Recommended Stationary phaseComposs to be separateTypes of spusedGasesalumina, silica gel,Leolites (mol sieves parousGas : solidpolymersnonpolar liquidsmethylsiloxanesPiBs,petrochenicalpherylmithy/silorows, polysiloxare carbonarhurbicides/poricidesphenyl polysilpherylere siloxaresGas:Sugarcyanopropolypheryl methylsi branesfree fattyacids. alcoholliqpolyethylere dycetsalcohols, aminespherylmethys siloraresSample InjectionA Sample portinto oducing sampleCarrier gas of required to flow through the injectorand push the gaseous components of the sampleonto theGC Column which leads to the detector.It must be dry, free of 02 Ey chemically chirt mobilephase. Hecommonly used. becauseit is safer than H2N2, Ar, H2usedLarge range of flow ratesdepending upontheCompatible with many detectorsdesired performanceand thedetector beingused.Column oven :-ThermostaticOvenserves to control the temp of the column within afew tenths of a degree to conduct pricise work . Can be operated in 2 marrers-/Sothumal programming - Constant temp throughout--Tempuature programming - (graduint)works best only if the boiling point range of sample is-nallow-Detectors (bu)-- After the imponents of a mixture are separated using gas chromato--graphy , they must be detected as they exist the GC column.It is located at the end of the column which provides--a qualitative measurement of the components of themixture as they elute in combination with the carries-gas irc DetectorsThermal conductivity everything 10's of ng not very sensitive, easyFlame ionizationhydring carbons sub-ng very liver , relatively easyto operate, only one gasto operate, required fuelgasses, not sensitive.Nitrogen phosphorus . nitrogen 1 how-pg very selective hardtoSulfuroperate required fuelgaresElection capture Halogerated howpy very sensitive, radiationnitr 0Source not very lenearSelective 2 gaves.Mass spectionsteesAlmostdependsorsensitive, requires pumpeverything operation system fauly complicated11requires waring OFCKa therometer 1 hot wire ditectorFirst universal delector for GC.Processmeasures a bulk property of Mp leaving the column.11ability 2 conduct heat away from hot wireThumal conductivity charges with presenceofothercomponents in the MP0analyte appears000gasTCTempwire resistancedetector currentread outTCD compares the thermal Conductivities of 2 gas flows- -Pure carrier gas ( reference gas) cy Carrier gas plusSample ( column effluent).Detuctor contains a filement that is heated electrically Sothat it is hotter than the detector body.The filament temp is kept Constant while alturate streamof reference gas Ej. CO lumn efficient pass over itwhen Sample is added the power required to keepthe filament temperature Constant changes The powderdifferences are measured and recordedwhen helium (or hydioger) is used as a Carriergas,theSample causes the thermal conductivity to fall. if N2is used the thermal Conductivity goes up bcot mostthings are more Concludive than N2.Tep doesn't destroy the sample during detection.This car be be connected to a FIR or other detector.