Sample Preparation

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

285

Pages:

4
Author:

Amal J.

sample Preparation:samples must be prepared using Solvents that containdeutrium in placo of hydrogen.conc range from 5 - 25 mg per ml.samples should be completely disolved , no solid particlesuse internal standard. magnetRFTransmitt ofdetectorabsorptionmagnetController> Bmagnetic field.Application:-uses the principles of nuclear magnetic resonance to image trive.The dynamics of proteins and nucleic cuids over an exceptionallyaudie range of time scalls ranging from seconds to pico seconds.protein ligand interactions under physiological conditions by Simplyadding ligand to the NMR sample of the unliganded Samproteinatomic level structural info of proleins and NA in soln(2D NMR)NOESY - distant dependent coupling of Spins in nearby atomsthrough space.Tocsy coupung of spins in atom connected thow bondsAway from negativityrupfieldNear -11downfield identify the Carbon - hydrogen frame work of on organic cmpd...works with certain nuchi ( either with odd no. of protonsor odd no. of neutions)136 - no. of protons = 6I3elections.'Hmass of 1 11 pro no neutions)no. of neutions = N 7-pro+ve charge, proterty - Spinmoving creates a magnotic field.000Protons havingwhen magnetic0random orientation field appliedBomore stable align with Bo/align2 spin state (1 overcrugy) against itagainstB spin state (higher errogy)less StableB E energy difference blw & and BBoAEa right amount ofif you add radio frequency energy to a proton , it car flipfrom the D slate to the B state. Now, if the nucleifalls from the B - b state 7 it emit radio frequency energyH takes energy Gy constantly flipping backresonanceNMR.when you put energy into H, you can promote it to the BE falls back release energy1-1 AE = hvV = [ X Bo]2ThHgyromagnetic ratio = 2.675 x 108 T 5-1 Mobile phase carries the individual Components to a cutaindistance through the stationary phase depending on their attractionto both of the phases (dolubility pactioning , affinity)The differences in mobilities, , sample componentswillbecome separated from each other as they travel through-theStationary phase.Detectionlobile phaseinjectionStationary phasedelutormobile phaseport(separation of miniture)Sample