Type of Atomic and Molecular Transction

University:
Swansea University
Course:
PM134 | Microbiology
Academic year:

2023
Views:

324

Pages:

3
Author:

Amal J.

Types of atomic Ey mdecular Transition.G*AnlibendoGr rags = NuclewTi*6AnlibodX says - Care level e-cUV lays . valuen/=Nonbondingvisible -value eit6Brindinginflared - Mol vibrationGBondingMicroerve - Not rolationsSpinRadiowall - Nuter Spriionizationconcreationcompten ScatteringxraysOOLanger wavelergthxray.photolonizationlevel changesE gap=EXNAUVIvisNol rolasvibNA = 6.02210-mal-1conizalinfracedMol rotation & torsion.minowave2b 33 +9266.626 x1034 X 2.99 X 10 8-171]26450 X 109 = 2650.44810J E gap = EXNAintensity opinionenterples ofTransmittance T= 10 / ItPlodiationItrediation0/0 T= 100 T= 100x 10IItAbsorbance = log 10 10 ItT= 10It% I= 10010/1t11= log 10 1/tA=log TOlolltlog 101/t11= log 10 100% T= log100% I t10= 2-log 10 % T= 2- 109,0%TConjugated double bondscarboxyl groupsbestabsorbers in UV-Vis range.Carbonyl groupsNitro groupsalomatic hydeocaebons absorption band at 2801 nmdue toItTC transitions.The region of dutiomagnelle radiation that is not absorbed issimply transmitted reflected back or Garemitted through the matter.TheA = Ecd0.48 = E X C X d= 2200 XC :I0.48 - C2200A 100sooAto2002 04 0608Concernation02040601PattlengthpathlengthcmLimitations of Bur lambert law :limited by chemical & instrumental factorsDeviations in molar absorption coefficient at high concenturationdue to electrostatic intuactions blw molecules in close proximity.Fluoreena or phosphorisence in the Sample.charges in the refractive index in high analyte concertuationScaturing of light due to particulars in samplecharges in chemical equilibriumcuretteDeutrium and hydrogen lamp (UV)Plasticw filament lamp (vis)GlassXe are lamp S. CUV and Vis)Quantzcalnademutgas-QuadzwinderedAnodel+appd haped cathodeTungster wire act as anodeClass jacket filled with Ne long aurt guesat a low premiue500-WOOV - is applied b/w 2 eat ans of in ort dos is conized