Examining SGOT Level and SGPT - Edubirdie

University:
Massachusetts Institute of Technology
Course:
HST 198 | Health Sciences and Technology
Academic year:

2024
Views:

93

Pages:

2
Author:

mason mc44

PRACTICUM FOR EXAMINING SGOT LEVELS AND SGPT Transaminase enzymes are often used in assessing liver disease is: a. SGOT (Serum Glutamic Oxaloacetic Transaminase) or AST (Serum Aspartate Amino Transferase) This enzyme is found in the cells of the body's organs, especially in the heart muscle, then liver cells, body muscles, kidneys and pancreas. SGOT mostly bound in organelles and a small part in the cytoplasm. GOT got it increased in liver tissue damage disease, whether caused poisoning or infection due to viruses. b. SGPT (Serum Glutamic Pyruvic Transaminase) or ALT (Alanine Amino Transaminases) Practicum Tools and materials Ingredients: human blood, reagents. Tools: syringe, spectrophotometer. alcohol swab, test tube, micropipette, zentrifugen, clinical Work procedures 1) Blood collection Approximately 1 ml of blood is taken (without fasting or with fasting), then the blood was centrifuged at 4500 rpm for 15 minutes to obtain blood serum. 2) Measurement of SGOT & SGPT activities Take the serum (100 µL) and put it in a test tube add SGOT and SGPT solution (1 ml R1). After that on vortex and incubate for 1 minute. Then add 200 µl R2 then vortexed and incubated for 3 minutes using a photometer clinical varta 506. 3) The data obtained is the difference in average absorption per minute (ÿA/minute), sample measurements and a correction factor of 1746. Enzyme activity (U/I) = ÿA/minute x F Evaluation a. Experiment Results Enter the experimental results data into the report table b. Discussion 1) From the practicum data, interpret the examination data. 2) Give several examples of diseases related to SGOT levels and SGPT

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