University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

202

Pages:

4
Author:

Amal J.

EFG-GTPPeptide @ Asiteuncharged tRNA @ Psitesmaller subunit anticlockwise dire notationhybrid state of ribosoncea shift for peptidyl region of the tRNA go to the subsequentsite(opperregion)notesactly bound)aanEFG-GTP binds toshitFmetshyA and hydco lysis of GTPby FBGanticlock997G-GDPinformeswikefinetTotatoo2unlockGTTPelhosom2bind Highlyto Asite 1.There lateral gates in b/w A&P site P&E,,whichcan be opened, in normal it in closed, EFG-GDPopens the gate Conbating of ribosome)2binds to A site and push the tRNA from A toPsite through lateralgates.As the tRNA moves it takes mRNA along with it,next codon is thus placed in A site.now the hybeid state in destabilized , G EFG-GDPleaves the site1opening of lateral gates.2Binds tightly to A site215'3'3'GDP5'GDPopenedclockwiserolationgates&destabilisationSto AsiteS S3/5"GDP Release factors (RF) - structural numicfaminoacyl tRNA.class I (RFI),class II (RFII)Just likeERNA, specialseq toRf3RF1RF2recogniseterminationUAAUAAcodons.UAGUGASaineProtinePeptideProlineAlanine/valineanticodonPhenyl.Thricninealowattacking group.(SPF).(PAINT)NH2NH21Glycine Glycine GlutanineGAGC(GGQ motif)QCGGQmotifhydrolyse pepticleRFGlyGly Gm)fromcomplexSPF/PAINT (Peptideonticodon Recognisestop codor. class IRecognise stop codonRelease Polypeptide from complexGGQ interact with peplidyl transferase centre of505 and induce hydeolytic activity and Peptidytransferase now remove the pepticleCCA - has some forctionnot elucidated untiltRNAnow.Class IIRf3 helps release of RFI and RF3 (class I).in GDPform RF1&RF BactasWatsonRF3-GDP.4 GEFreleaseItdirectly pull ERNA from the sites tocytoplasmRRf, EFG, IF3@Asile GTPbinds to E site offormRibosome30S and prevent ribosomeRecyclydingreassociationfactorEFG as soon as it in releasedSeperateeironomalinto cytoplasm, it in selfsubunits.capable to replace GDP with GTP