University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

50

Pages:

3
Author:

Amal J.

Factors for lolyacongratorsCPSFcatalyse cleavage & PolyadenglateCleavage Polyadenelation Specific FactorPAPPolyA-pdymelasecleavage & addition of 200-250 polyadenylic acidresidue @ 3'endCFI- Cleavage factor- 1only required for cleavage,CSTF - cleavage Stimulationthey leave after deavageFactorsymplekin - scaffol to hold othercomplex (stainization).(Cleavage & Blyadoxyblen)PABP2 - Poly A tail Binding Protein-2, 1 A PABPfer 12residueprotectionaddition of PABP fastens PA PactivityPABPN(nucless)middle.(PABB)PA PactivitySpeedPABB E(Cytoplasm)time(PABP1)PSUrich.EEPAEYeastAACAGAxUAUA10- 30 nts afterPAEAAUAAHumanCACA Termination-ve+velongationtermination@@factor when2 modelsPircasivity of enzymedecline end slowdown.5'-3exonucleaseTorpsido model.Allosteucmodel Cantiterminator5'- 3' exonudeasemodel)human-Xrn-2:2possibilities.Yeast- - Rat -1IPolymerase IIrapidly cleavelost anti termination/5'-3'of RNA collideactuallywith RNAPOl and+ve elongation factorbothdecreasing processivitymodelsthey detaclsof RNApolIcombinesynthesis by BIIIinterminationslowafter poty2allosfacic terminatingadenylation but 5'-3'factor consinalcleavage rapid+ve elongationXrn 2 & Rat-factors andSteaveand detachpotcause allosteric modification.niRNA transport to CytoplasmhnRNP - in nucleus (mRNAtmoteins)cap hands to CBC-20/80 complexExons splice-junctions have excon Junctioncomplex - EJC (20 - nts awas transpece).Poly A protected with PABP2.hnRN Ptronsportedthrough NPC withthe help of NXtnucleus holsome remainssome proteindegratte inandNXfThe hulky boRNP require2 proteins NXT,Nxf. NXI4NXF DIDAS TO MRNAmove throughNPC (Nuclear pore complexCBC-20/80 - ,NXT, NXF and PABR disociale bybp-5the helicase activity of NPCIncytoplasm PABP2 in replaced by PABP1EJC - Exon Junction Complex, in every excon bfr20 spliceNXT - Nuclear Export TranporterJunctionExpertinsNXF - Nuclear Export factorNPC - Nuclear Pare complexCBC - Cap Binding Complex $20/80 - -heterodimer Jones20/80CB20/80, NXT,NXF, EJC & PABP2 are dissociated intocytoplasm and and the transported to NPC through NPCwith help of importins