University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

220

Pages:

4
Author:

Amal J.

3 stepsIntialionElongationTerminationtRNA charging .amino acyl tRNA synthetatase12adenylation t FRNA chargingaminoat ATPcharged aaChighenergymokeure)B&HHofi(1-CH26HoAH+-N-Cand+T1HHI11hadHb/wHOHOHR04BphosphPyrophosphataseenergy.cleave thePPI2PbondPPilPyeophosphatase2Aenergyused fourcoopling of M Adenoiscof aaHisP+H3 N-CC-O-P1hyR0-CH2Adenylated^mixedAaaanhydride handtypes quid) LKNH cnauguraa from adenylated adenylylytated aa to3'A of 5th tRNAall61t2$amino+AMPATP2ADPrecyclingIfif15th - -CN-C+ O-P- 0k'0!!1C0- CH2KitOHOHt RNA synthetaneclass Imonomenicclass II di/tetrameucaa toaa to 3'OHbut finally on 30H2'OH of3'Aof 3'A of tRNAbcs ofof tRNA.PeyehydrophobicPrejer hydrophilicstelicsmall aabulkyaahindrance.reaction equivalent to 2ATP hydrolysisstepIhydiolysis1ATPAMP + 2 PPi2ATP-2ADP+2PiPPi2PiRhydiolysisequivalentstep2released AMP +ATP2 ADPfidelity in dependent on synthetase 20 aaeach amino acid has specific synthetaneBactaia100 synthelase for G/D chargin, butthere in tRNAGlnthey use Glo tRNA synthetaseglu tRNncharge tRNA of G/n with GlomodificationGin., 1it+1H3N- C - coo143rd- coo1-CEl2C H2CH2amid OCH2.transfeiarecooC=ONH21glu + tRNA + AIP glutRNA glotRNA gln + AMP+PPiginsynthelaseginglutRNA + gth aminotronsfac ghntRNA ghnATPADP+Pi Selenocysteineno ERNA synthelate.ESecsertRNAsertRAsecGsec(Se ERIVASelenidegroupSeleno phosphateHse synthelaseHse - PATAMP+PIseleaophespateSertRNA Sec + Hse -PC.H2CH2+PSelenoICysteine OHHSesynthaseSectRNASeePyrolysinealternate patway using lysinelysine + pycole ringlysi lystRNA + Pycole ringPlyPlytRNA Ply.