University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

204

Pages:

2
Author:

Amal J.

LacOpeionPromoter (transcriptionstart site)RBS RBS RBSNAiPstructuralregulatory generepreisoroperatorgeneprotein)chindingsite ofrepreser)-veRBS - shine-Dalgeino Sequence/RibosomeregulationBinding SiteZB. galacteridaseYLac permease [facilitate transport of lactoseinto bacterial cell]ATransacetylase [deloxify the lactose analogues]IPTG - Isopropyl ThiogalactosideGratitous indicer- used to study lac operonIPTG carrofbe metabolised,Even in the absence ofit permanentlyremoves theLactese there in a base linerepressor moleculebonscription). Ato-lacture in dynamin binding andthe actual inducerCactoseB1-Hleaving of representAlo lactoseB1-6changeThisconversioncatalyzed by B-galadosidase&permease TS required to bringhactore inside when high amount of Lactore in prement its inthe cell then only high amount of Atolactore willbe produced, then only foll repression can hereleived10,00,00010Alo-lactorelactosenot C soughtoadivate1,00,00,000100EneughDiuxic curveSugars enteringlactoseintocells arewhen mediumimmediatelyswitchingcontains glucose andglucosephesphcrylateSigmoidcureInducedlactere glucose is(Lac+gal) ALLburning /cakthepreffered sooree (7zy/ is theboliimefragasmore genegrequiredsi nople sugasTo trap thefor lactose utilization) Repressionglycotylesugar in theof lac gene expression impresencepathcellof glucose in called catabiliteglucose it starts switching to utilizerepression If medions depleteImprotacystealactoseThe growth curive inPhosphalecalled Dioxic Curve-Transfer SystehelpsinPhosphate Transfer systemphesiberfyPEPE1HPrEDAEteTropp 679,680If glucose is present inGlucoseGlocase G (P)components are dephosphonylatedemop gh amount all thereEIA in dephosphoylated state block Tax permease transporter. 36