University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

228

Pages:

3
Author:

Amal J.

-in muscle30,00000Translationx110KDa= 33,00,000amRNA, ERND, aminoa, ribosomes15" Euror rate 1/10,000anloy.& mar than 2.5megal Dalton proteins isribosome2.5MDa abighlyconsecusProteinProkaryotemachine coprotein5+23GosEukalyste size3450sin kept5+2815.849small toEPAkeep21translation30s33. accurate40s70s161880S.RNA contribute most55,165, 238555,5.8,18,285.of the mass than proteinavg md weight ofsiboxamal proteins55,5.8+281855+23165--15KDa305lengthprotein euroratGOOS40505small proteincuorateso proteinsizekept snallDecoding centredistance b/w 2rihromessonts.anticodon - codon interactionsmall subunitPeptidyl transferase centrelarge subunit RBS - Riboxme Binding SiteShine - Palgaux sequence3-9 bp upstream start siteRBS has complementary seq on subunit]hiadsfirstanti-shine dalgarno seqaBasepairingvery important:Polycistronicnormal Each ORF has start, stopf RBS sequencesORFbut some lack RBSAThe ORF in then translatedby translational couplingcoupling of the translationORF without RBS with thepreceeding ORF.startstop start-RB SOR FORFRBSXsicutmyWas/pikeAUGAback stracking ofstopriborome to red AUG after UGA Capping increase trouslatabilitycap& tail act as signal of translationrihosome find on Cap f CBCtailInitiation factorrecruitment3++4EUK mRNAN/GNN AUGG-3----- +1+2+3+4Correct AUG flonted by Korak sequenceAC C AUGGallskip AuGis and select AUG Hanked by Kosak sequereand this scanning in called Leaky scanningtRNAA3'distructure5'aminoa acceptor aim3° L shapeshapedelover leajDeoopvariable TCC 75-95 nucleotides..Anticeoodon343536(kasak 5' ANNAUGG3Biggest tRNAcelenosysteine.