Trombone Model Tutorial Materials

University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

347

Pages:

4
Author:

Amal J.

diiReplicationConservativesemiconservativeDispersive000++Meselson Stahl ExptC3.Isotopes of N.15:14NH4C1NH,alightKSucrose velociteheavy .gradient Condivi15NHHCIbookJugation to0th genseparatedyCulturedin HNMediumsized gessCCB0.25M S14 NHACI1I genE-odil0.5MnoneIMSUSS e29 wanCsclwas wax work was AYX AAA xxx myContriguation PhologenateddensitygradientLightcentrifogatorInterto seperateheavy&light=HeavyDNAJ,SSCI&DNAothgonDenature IIIIdenstryIgongengenin comparattAfter 5th generation = 2'-2 (negeneration)so no : of light DNAchainafter egeneiation = 22-2= 4-2 -2Bacterial Replication5' 3'lightlight.heavynoheavy120/923in bothradiohighdirection3'5'activeopermediumreplicationbidirectionalindicateslight:with lowspecticaDreplicationrollingcycle replicationH3, BrobliaBacillessublites. Oric - - 246 bp ) 5 Amerf3-13mc sollice mofifs, A-Teich1DonAboxoii)IIIIII2DnaA Recognize OriCendmell DNAbyA Phydiolysis ,HURFIESPSSBP (inhallybirding slow (cooperative bindingdo one SSBiniver poressB) home5DnaB - DNAnchiase (Atphydolysis tomelt the DNA), hexamenicTlagging strend!proteinDnaC - Helicase loader4only throughChobramDraPolymerase - DNA strendsgathesplaggingRepeatedprining/Primase (Dna G) - helicase speinase activate mutually bothmove together prime synthesis.Topoisondias - Removal of supercolle, RNaseHg ase - NicksealesTey. Site -Terminati and Parmet replication (1) helicaset Peimaneopp to OriC>okazakimove together laggingstand needs continues5'3'priming&a3'botlata35(2)(newdna) okazakiLi. 3DNApolymers+ PPi Pyrophorphate(d NMP) + dNTP(dNMPntlPycophosphatase2PiAll macromolecular synthesis( AG = -7-kcal)driven by the hydiolysis ofgive energy to duve the reactionPPi to 2Pi hypyrophrophatosein a a variable directionWhy ON noeed RNA. prima to start replication?developed in Absence of DNA androRNApdymeras DNA pot havePeof reading, activity.progreading DNA not later evdved, RNAndRNAPOl.DNA available and so ONiA requires an RNA primer forwas attachement of dNTPS and begin synthesis21 DNA Pol - I, II III, IV,VPeoofreadingImonomeric, 5'-3' polymerase, 3'-5 exonuclease xeroxve andadd incorrect correct-5'-3'd exonuclease, ReplicationPrimerremoval.II5'-3'pol, 3'- s'exonuclease RepairIIIMultimeric, (a) I 5'-3 pol, 3'- 5'exonuclease ReplicationmajorIIIIIhighlyexpensivelessPiocessivily:- no:d(lunit)nucleofides odd -efficientefficient .before detachemar'scatalytic1000 nucleotides/10 nucleotides/secondsecondhighproceesivity.lesssynthesise several1000 nucleotides20 nucleotidesbefore they dethacbIII amagonpolypeptidesCoret O - stimulate exonuclease activityPoly e E - 3'-5' exonucleaseage mex a a- - 35-3' polymerase