University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

271

Pages:

3
Author:

Amal J.

wobble hypothesis.3'mRNA.normal5' ERNAanticodonGCUcodon 5'-73'Aanticodon 3'- 5'A/G2UcluThe first newGnucleofides stricklyInosineC/U/A.(modified base)follow watson-crichpairing, 3' nucleoticalHypoxanthine - deaminatedofbase adenine.maynotfollow codon may or watsoconjugationIf 'CAG'Gickbase pairingof ribose sugarenormalwobble3'Inosine3'GUCS'GUUSValine. If base pairing in stablewobbling fors' anticodonb'end infree butThe ribose sugar should3'jlanked b/w 2 nocleolidemaintain an optimumdistanceand they are notflexableso the 5 nudeotide conCasodago for worbling.C&A as 5'nucleotide of airanticodon they cont maintainthis nihose distance.Beauty of evolution - Geneti codeGenetic code in evolved in such a waythat the deleterious effects are neutralized(1) substitution mutation1 nucleotide in replaced bgonothermestcases UUACUAUUAAUAneutral.LeuLeuLeuIsoleucinehydrophobic aaSame aaSame groupneutralmutationnotgoingtoaffect the functionunlessonduntil it'sa critical residue Protein folding determines functionallhydrophobic aa rest inthe centrehyprophilic aa peripheryUCUCCU16.20dPurinehydeonucleatidephilicSerProaa-PysimidinehydrophobicXaadeleteriors.subs motationtypestransitionPransvessionPyrPyaPysPurmore hamful.PorPyrPurPur.freq.freqmost mutagens are transition causingAspergillus flavusaflatoscintransversion causingagentburned meatchemicalhydiophilic expelledthroughurine/deroxified tohydrophilic and thenCUUCCUexpelledanother product will behydrophobicformedbenzopgeineReduced badeffects.transversioncausing agent