AMES Test Lesson Notes | Swansea University - Edubirdie

University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

143

Pages:

2
Author:

Amal J.

AMES TEST - detect whether a chemical cause mutation & type of mutation it cause Salmonella typhimurium - auxotrophic strain (His-) → ? chemical cause mutation If chemical is a mutagen they can convert S. typhi → wild type If benzopyrine in chemical to be tested → in this form they are non-toxic Rat liver microsomal fraction extraction + benzopyridine ↓ benzopyrine epoxide → test → Type of mutation 3 types of auxotroph mutated by: → transition → transversion → frame-shift → chemicals1 +Rat lived: 1) auxotroph by transition 2) auxotroph by transversion 3) auxotroph by frame-shift → chemicals2 → transversion

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