University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

416

Pages:

4
Author:

Amal J.

Damage RepairProkaryotic repair system- cell cell try to repair @ minimum cost.(1) PhotoxeactivationDirect Repairlight dependentblue spectromvisible lightacceptphotolyasepyximidine dinnerbinditodimaindarkBreakcydobutone ringphotolyaneMTHLuse 2cojactorsfor blue eightmethylene Tetra Hydeo foluleahsor ptonFADHexcitationenergy (electron delocalizationin cyclobutane ringBreak the fond.Gyptochrome evolved from photolyasephotolyasereverse damageDamage reversal process. (2) Alkyl transferase enzyme (ATE) Damage reversalRepair Alkylation damage of DNAAlkyl Transferase 1Alkyl transferase2(AT2)(ATI)Cysteineinactive siteSHwhen ATE encounter an alkylated base,the altyl group in breaked and joined tocysteine residueInactivatedSACH3 SHafter IATECH3AroundofCH3ferenceremovedalkylgcoopwillbeconjugated foreverSuccidal enzymeInactivateAT2suicidal butAT1Transcriptionalnogeneregulationmainactivatorenter in theenzyneof A.T 1inactive state ansenceof AT1 Alk BIf 5methy ajtosinedecardinationThyn.remove damage fromG111 methyl purines&3methyl pysimidinesglycosylasereplace Twith CExcision RepairPOHBifunctionalPZ01/4S- A = Tglycosylase.SPDBaseExcisionS-G-GES2Repair (BER)PPPPSS.112POHP32SGUSGAperinicsite (AP)UracilDNAPglycocylase(break glycosidic bond)45P.PPhosphoS-GAPendonucleas.SdiSS-GP(5'site)esteraseRxIremoval IPolymennaIILLAS GECBaseaurec G=Ashouldaddexcision repairGremove A8 & & replace CogniseKrepaired by normal(BCR)-oxocasNucleotide be Excision RepairFail safe glycosylase (Spct)(2) Incision 2nicks(1) Recognition of Damageon both sidesof lesion.(3) Excision: helicase(4) Polymerization &nick sealingremove damaged portionUVrABC escision excinuclease mediateNucleotide excision repairBslight belicase Chelps in scanning processCORRAUVTAbinds DsdnaScan lesiondimenizationbind to damageendonuclestyticenzymeconfirmationnicknickUVICchange of UvrBOur A release