University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

331

Pages:

2
Author:

Amal J.

InhibitorsEukaryotic transcription1. - amonitin2. ActinomyainProkaryotic translationInfiation1. Aurintric carboxylic acid - Prevent IF bindingto 30s2. Kanyamyain. - Insibit f-met ERNA binding3. streptomycine - Inhibits theformatt onsf intiationcomplexIE longation1 Tetracyclinbinding of amiroacyl tRNA to A - site2 Streptomyaincodon misreading & insertionof3. Paromyain.peoper aa- Increase warf Hundred rolein4. Kirromycin - Binds to EF-TO, preventingconfirmational switching of EFTU GDP5. spalsomycinpeptidy transferase activity(235)6. chloraphenicol - Binds to 505, blockA siteinhibit peptidyl transfelaseactivity. 7. Clindamycin - Binds to 505 subunit AfPsiteardpeptidyl transferase activity8. Erythromycin - -Binds to 50s subunitard causepremdure peptidyl tRNAdissociationTranslocation1. Thiostreptonribosome dependent EF - Tu&EFG - GTPase activity2. FusidicacidEFG GDP dissociation fromribosome3. Hygromycin T translocation of Asite FRNA toPsiteTermination1. Puromyainmimic the aminoacyl tRNA andbinds to A site causing prematore terminationEckayotic translocation1) fuscidic acid EF - G GDP disociate fromrinosome2) Diphtheria toxinEf- 2 through ADP echosylation3) cloheximidetranslocation of peptidy ERNA4) HygromycinERNA to PsitePeevent translocation of A site