University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

318

Pages:

3
Author:

Amal J.

Transduction1cosabedral - (20/paces)- -Bacteriophageneedinnerbollow cycholocial needle shapeTail covered by fail sheath.fibres -(6)contractileTailpins (6)binding with tailfrees fibres - reversiblerrevesible bindingInfectionTail pinly803ymecontractionDNA.eject.8&T4 phage libera 1300 progenies1from E.colilysozymetranspirImmedeate earlyDNase, RNAse protease3 Greeneshostcell proofDelayed genebijack bestLate genescellmarbiencyhead& tailsynthesize phagehycosing(IRES),operately stillgenome from hostDNAtobeadgenetragmentsassentile Tailsmeclification of phage geno mecytonine(HMC)(Mcys)hydroxyme thyl cysmethars HMaysGuscocylates Integration depends on IS -Insersion Sequenceelement403episome(OYLDi432Cg d edbC9ddeHFr [High frequency Recombinant]transfertof-ordersiaibISChisdce9 &gdePartialcontrolled interuptiondiploid (2 copiesof matingMapclistanceofagons)11TimeMelozygote - lackentive plamidb/w HFrf F cellDNA& receivesBacterial genome*segm entGenes TransfacedPlasmid insation siteStrain IYGFTYstrain 2PODCSstrain 3PQSYtake-GiftStrain1strain 4GBYEQFPStrain 5QPODstrain 6 TCDO-D-C QrHfr A - Bio (H)- GLO(20)- - His (27) - cys (37) -tyr(45)Hyr B xcl(6)- - Metasityr (24) -cys (32) - his (42)Hjrc - his(3)- y -cys(13) - tyr (21) - Met(27) - Xy(3)HfiD,xyl 7 -ttr(25) - lae(40) - bio(4r) - glo(62 TransferTI tyr E his(4) - g(o(ll) hlo(27)-lac(35) - this (50)(A)BioAas &16Gloi115thrHisdautheatONElovexcl pizCCysBiogonesMet G x/s8GluBhearestgenesHisCysTyrMetlacxythe