University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

352

Pages:

3
Author:

Amal J.

ParasezurescyclePARASEXUAL CYCLE - horizontal / lateral genetransferSexual RepAsexual RepAdvantageAdvantagesGenerate variations byTransfer foll geneticpotential tomogenyrecombination atmeiosisHelps to overcomeRely on Paraseswalfast evolving paranitesCycle to generatevariation.Disadvantagecan tranjer only half ofTransductiontheir genetic potential to thePhageconjugation.TransformationmediatedTransfer bynaked DNAprogenybacterialcelltotransferDNAcelltransfercontactTransformationFred GriffitB 1928Bacteria lyserelease considerable amt of DNA[only related DNAcompetantfragments transpered]cellscan takeup1 cellwall COMG, COMEACompetance factors2Plasma membrone Com FA, COMEC2 transport proteinsonly siongle strood transfered II degrade4cleavage of the IIrelease energy thatdrive thewhole processMediumcomfaComGSSBRecAl Scan forcomplementary600comEcDNA+ Protection]COMEACytosolcompetanceinducedio 2Transfer of HjrEpisome - Plasmid that have the ability to integrate intobacterial main chromosome.or remaincanremainintegratedDependent on Insertion SequenceElementindependentInitiating segment starts transfer of plasmidalong with adjacent dromosomal genesreceptent does not become ft since fplasmid in not folly transferredPro law43oriof TransferOweHfrFtcellTransfer to F-RacPrayOFrecombinatFcell.3Transfer of fplasmid - Sexduction or F-ductionFplasmid + bacterialetromorome integrationDNA of plasmid + dromosome can be seperatedf plasmid + segmentofotromossomeFplanidFstrainF Fwhole F is transferedPartial diploidHetelogenoteMerozygote FjactorFjoctorF cellF'cellF'cellHFrStrainGene transferedYGF T+Y1SGPODC,2pasy.3FGYSQ.4xO+B5QPOD,TDC6TCDO.Hfr. A - Bio(4)- - GW (20)- His (27)-Cys(3+)--yur(45)HfrB - zyl (6) - Met(18) - -tyr(24)- cys(32)-his (42)Hfrc - his (3) - cys - (39)HfrD - xyl (7) - - thr (25) - lac(40)-bio(s)s) - glu (G.2)Hfr E- - his(4)-gh (11) - bio (27) - lac (35) - thr (50)ABiolac16 GW157 x cthrHisB1810 YExyl12Cys&,nMot C