Irreversible Inhibition Study Resources

University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

50

Pages:

2
Author:

Amal J.

Irreversible InhibitionSubstrateGroup SpecificSuicideAnalogsInhibition-interact/modifies- molecule structurallythe groups of aasimilar to the sohstratepresent in the activeand can hind to the activesitesite.Group SpecificEg : . DIPF Di - Isopropyl Phospho flocritatemodify critical seline residue in the active site ofenzymes like chymotrypsinsplit to Acetyl& eholine mediated by acetylcholine estelases comeAcetylcholine - does not come back intact to neurons, to theyback to neurons.DIPF modifies a Ser residue in Acetyleholine esteraseso acetyl&choline in not generated leading to a continous signaldischarge - leads to Paralysis.Aspirin (Acetyl salicylate)Aspirin block enzymen required for synthesis of prestaglandins.Piostaglandins are responsible for fever, pain etc.Prostaglandins (PG) are made fromFatty AcidsPLA2linotelicicArachidonicacidacidCyclooxygonasePGG2I Peloxidese.Prostaglandin -Vo Vmax it's alineas graph,Allosteric Enzymesunlik M-MhypetMultiple regulatory /active sitesAllostericsitecurve.binded byEffectors / modularthe hoadivalor/Effector.activatermultiple subunits.iveeffecter't +vevcThey show cooperativelyallosteric+Binding to one active site modifies other active site.inhibi[binding of O2 to harmaglobic in co-operative"They show a sigmoidal curve because of this.InhibitorsSmall molecules or ions.(Reversible)InhibitionInhibitors bind loosely to enzymeIrreversible- Inhibitors bind tightly to enzyme.