Membrane Insertion of Proteins

Membrane Insecution of proteinsmembrane spanning domain - 20-25 bydroptobic aa isbelix which makes it emergentically favourableato be inseted as the membroneall the membrone proteins that are to be tangeledvia the secretary pathway are first synthesized on the ER membroneand their orientation at the time of synthesis will beconserved till they reach their targets.AB.LCLTopology[CL]cylosolidic sideNHATcoo",NH3+000coocooERmembroneNICOCN13&3TypeTailcoorTypeanchoredNHItype Nlominal/cleavableNHithN turninsType IIGPIExoplasmicsignaanchoredspaceABN-C N-LN-terminus facing side no: of membrone spanning domins- Membrone topology" Topology of membrane proteins means the the no: Nand of memb Cspanning domain it has and orientation oftaminus of the membrane proteins"classificationType I Type II Type III Type IV Tail AnchoredGPIanchoredLDL single palss.Cytochromemultipars.ProteinsproteinsreceptorP450SeckInfluenzaClaminusGPCRvsnalesGlycocylfolominal side drug deloxification ABCpamp&TPhosphatidy!HA proteinsnalesInositol (GPDInsulin receptorAsialogiyogproteincoo failGrowth HarmonereceptorNfaceanchoredProteinattarbed Transferin protein that transports iron in blood cells take up this T2Transferin bond to iron.lamb2 types of topogenic sequences - helps in orientation of memb'proteinshigh offinity to memoraceStop transfer ancbor (TypeI)Signal Archar (Type II & Type III)Sequence (STA)sequences (SA).movethrough the channel, they laterally move& beplaced(TypeI)(Ohon themesASignalSTApeptidoreNH3+sequence(membNH3+spanningNH3+Nt3+NH3+cleavedgomainsignalsequenceType II & Type III nocleavagle Nterminos signal requen, membspanning domain act as the signal.Signalanchor sequenceact as signal for anchor & transferSA+vely charge amino acids (astreich of +ve charged aa)N+ve charge aminoacids either or N terminus /CterminuscDeterminesthe orientaintionof the Transmembroneprotein.whichever side poces + vely charge anino acids, That sidewill side not enter ER women, they remain on the cytosolic TypeII 1NH3+23N113+cytool+ve charge sideSAmethorove -vely of SAsequencesequen-chargedcefaces cylorol.ERlumon.cooType III312cooribosomemachiencycomes totarsloonNH3+only whenNH3+NH3+SA requenceemergeduringtranslationTail Anchored proteinNH3+NH3+coohydeophoticATP-gel3Etaminal tailATPGet3ADPPiN113+ADPGet2Get1 Type IV AAllanate SA&STA+vestretch @ NH3+Type W-BSA, SA, STA, SA,STA, SA, STAGPI anchoredGPI anchoredproteins easilyGPImove throughPM.Hydiophobicusual transmemb proteinPolarNH3+attachements, GPIdonothave cyto 8 Keletalhave it.GPItransamidasecoocleaveNH3+luminalside(Perform GPlancher)Hydropathyplotgenerated through compoleiprograms.Topology study of protein+ve valuehydrophobic- -ve valuehydrophilic aaType ISignal sequence (N terminos)+veSTA sequence +vetwo peaks at positiveside#14SSSTA TypeIISA sequence+1o-1Typertransmenpronedomain0-1Cytosolic Pathway609,3-1RemovalNature ofTargeter organelleLocationseqof scoofsear6-12 hydlophobic aa-ERlomenNYes.preceed by 1/ mor e basic aa-Amphipathic helix,NYesMtmatrixiYesrich in Sa Thrf smallChloroplastNhydeophobic residesstrome aPTSI (SKL)@Cc-mostNoPTS2 @ NPeroxiromeN -few(matrix)short segment rich inNuclesVariesNoLys and Arg.NucleoplanMitochondriaEvolved from Ricketsia prowesti - theory, DNA- - some of the proteins are synthesized by nuclearTIMTranslown of Innel mentImport poreTOMTrmislorian K/ Jonet outer man