Notes on Different Regulatory Strategies of Enzymes

University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

319

Pages:

3
Author:

Amal J.

Regulatory Strategies of Enzymes4 principle ways to regulate Enzymes1) Allosteuc control2) Isoenzymes / Alloenzyines3) Covalent modification4) Zymogens1) Allosteuc Control.- cooperativity, sigmoid arveEg:- Phosphofructokibase2 sites.I ATP binding1 AMP bindinginhibitinhibit activateglycolysis.Glyeolysis.2 Pysimidine biosynthesisCarbonyl Phosphate t AspartateCarbonyl AspactateApart transCaitomylas (ATCase)2sites erropN phosphonoacelyl L-AspitatestepPALAlast product inhibitorCompetitive in hibitorATPof ATCase.noeffecterATCase(Reohonit)2C3 3R2CTP6(2x3)6(3x2).regulatoryCatalytic.00 00 0080modulatoractivesitebinding site2) Isozymes /Allozymes.- multiple forms of an enzyme- vary in aa composition / subunit assembly.Lactate Dehydrogenane(LDH)280bunitsMHpredominant inpredominantin Heart.muscle2M2HMH3 M3 H4M4HH HMMMH MMMMHHNH.HHHHM Mheart1PyrovateRBC, kidney,is the substrateplacenta etc.(more affinity)aeopicaneorobiccondition.condition - A molecule in coralently attached to enzymetomodify it's activity.Eg:- Phosphorylation:-some enzymes are activated, come aieinactivated.active in formGlycogenGlycogen PhosphorylaseGlucoseGlucose Glycogen Glycogen.synthaseinactive inPform.Acetylationbreak interaction blwdistones& DNAat time oftranscriptionlysine transferse. AcetylRemove +ve changebinding breaks.Myristrilationmyristic acidIHC saturate fattya-attached to N terminal glycine residue of Enzymes.eg:- ARF G- protein in myristoilatedSRC in an oncogene desived proteinmyristollated.Fannesylation -(donely in Ras protein) in bioryntheticFarnesyl pycophosphate involved tapenepathway.Acetyl COA + AcetylCoAAcetoacetyl CQA + AcetylCoAHMC CoAI step of cholestrol biorynthesisMevalonateIPP(indole Pycothophate)