AER Experiments Study Notes

University:
Swansea University
Course:
PM-133 | Evolutionary and Molecular Genetics
Academic year:

2023
Views:

140

Pages:

5
Author:

Amal J.

AER Expense (6).Remove AER (nofgf8)Progress /pndifertiate+ feedback loopzonestopsMERAERsempvedXfGF8himp development halfSurfaceectoderm2) Grafted extra AER onexisting AER.extra structure (super homey structures)wings get duplicatedTowads the dital end.extraPERduplication of fingers @ distalend due to high amount ofnormal AERfgf8 (Ant - Post axis tightlyregulated by AER).3) heg mesenchyme placed beneath wing AERwing AERwing develops but @Legmosenchymes.distal end hidlimbstructure develops.(fingers replaced by claws) all abnor malities happen @ distal end becausetowards the dirtal end signaling in complicatedAER&merenchym controls the development(4)Distance is very importantPlaced leg gmerenchymeawaytion wingAERdistance increases& +ve loop instantingnosignalling, normalwingfgfs in not highly differinldevelops5) Limb mesenchyme removed & placed non limbmesenchymenonlimb mesenchynienorigoalingno limbdevelopmentnotcompetant enough toreceive signals from AERow our6) Removed AERfgf8bead dippection fgfrOoAERX00normal limbnolinbfgfsdiffores & reach mesenchymal limbcells & fgfs is required fordevelopmentAERAnotherfgf fgf4 made fromAdds upthe fgf8 effectSeveral especiment with different aged AER& mesend2groupsOld AER&mesenchyrYoung AERf mesendsymeIf AER in responsible for limb skeletal structure specificationormesenchyme youngA AER - in your our, MER4mesenchymemesenchymes very young .Iexptyourgmesenchymeremoved young AER&placedold AERnormallimb.IIexptremoved oldAER&placed young AERgroup ebscriAppt7pt writing129HD stptdevelopmentProduced the remainingpart.AER in not responsible. limbskeletal axis specificationAge of mesenchyme determineswhat structure shoulddevelop.no stylopod, zeugopod & autopoddevelopedyoung mesexchymereplaced hyoldAbnormalmerchyme blostima-woodepidemis formedMongmenenchymewhen lipphypeoutold mesenchymereplacedwith young.AGE of mesenchyme determines the proximaldistal axcis of limb skeletal structureAERdetermines Prox-dist axisAge ofmesenchyme prox-dist axis of limb skeleton.2 opperig gradients of moleules in proximal & distalregion( Lizard tailugeneration)Retinoicacid (RA)Fgf8duetopressereof AER &mesenchym.ProximalDistal.Intermediate cone