HEMOGLOBIN EXAMINATION Hemoglobin (Hb) is a protein compound that is rich in iron causes red blood color. Therefore, hemoglobin is also called a dye blood. Hemoglobin has the following functions: 1. Regulates the exchange of oxygen with carbon dioxide in the tissues body. 2. Takes oxygen from the lungs and then carries it to all tissues body to use as fuel 3. Carry carbon dioxide from body tissues as a result of metabolism to lungs for disposal. Determination of hemoglobin (Hb) levels includes: a. Physical method: comparing the specific gravity of blood to the specific gravity of CuSO4 b. Chemical method: measure Hb iron levels directly c. Colorimetric method 1. Visual (sahli): based on the formation of acid hematin compared with color standard (10% error) 2. Cyanmet-Hb: formation of Cyanmet-Hb (Hb is reacted with drabkin solution Kferisianto / KCN) Practicum a. Tools and materials 1. Materials: Cotton, alcohol, sterile lancet 2. Equipment: Hemoglobinometer (Hemometer) b. Work procedures i. ii. iii. iv. v. vi. vii. viii. ix. Add 5 drops of 0.1 N HCl or up to number 2 in the sahli tube To take capillary blood: clean the finger that will be pricked with cotton alcohol, let it dry again, hold your finger and press a little so that it hurts reduce Then pierce with a sterile lancet quickly, in a direction perpendicular to fingerprint lines The puncture must be deep enough so that you don't have to squeeze out the blood because it will dilution occurs and causes errors The first drop is removed using a wad of dry cotton. Blood drops Next, place it on the object glass. Suck the blood on the glass object with a sahli pipette until the line marks 0.02 ml / 20 µl in pipette Flow the blood immediately into the bottom of the tube until it is clean and there is no blood remaining in the sahli pipette Mix until the blood and acid turn brown (hemoglobin is converted to acid hematin) ix. Add distilled water drop by drop while stirring until the color is the same as standard x. Color equalization should be achieved within 3 – 5 minutes xi. The tube facing us is the one whose lines or scale are not visible xii. Normal values for men are 13 – 16 g/dl, women 12 – 14 g/dl. Evaluation a. Experiment Results Able to read Hb examination results correctly, accurately and precisely. b. Discussion    From the data and results of the examination carried out, analyzing the Hb results can be concluded Differences in Hb in Normal Women and Normal Men. Report (see Practical Result Report)