Enzyme biotechnology and industrial enzyme production exist in our daily life and. In many cases, commercial processes first utilize naturally occurring enzymes. However, this does not mean that the enzyme can be utilized as effective as possible. With time, research and improved protein engineering methods, many enzymes have been genetically engineered to be more effective at the desired temperature, pH...
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Introduction Energy, the ability to do work, plays a key role in all living organisms by providing its cells with fuel to function properly. Because of this, metabolism also plays a key role in all living organisms. Metabolism measures all of an organism’s chemical reactions. One of the main functions of metabolism is to organize the energy of the organism...
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Bromelain was first identified in 1891. Vicente Marcano, a Venezuelan chemist, isolated it by taking pineapple and fermenting it. Only one year later, in 1892, more research was conducted on the enzyme by Russell Henry Chittenden, Elliott P. Joslin, and Frank Sherman Meara. They named the enzyme Bromelain. They discovered that Bromelain is good at dividing proteins such as the...
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Abstract Garbage enzyme as additive in synthetic grey water treatment. Fermentation products are kitchen waste, water, Brown sugar. Reduce the pollutant in grey water, used for various household purposes. Garbage enzymes also have antimicrobial activity. It is an eco-friendly biotechnology approach to waste water treatment. Introduction Industrialization revolutionized the world as it brought along with technological leap. Due to change,...
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The effects of changing temperature and pH conditions on the activity of the β-galactosidase enzyme and its affinity for ONPG were studied. A standard curve was developed as a control, and was used to obtain the assays for the 6 different reaction rates studied, corresponding with the 4 temperatures and 4 pH’s. The reaction results were measured for its absorbance,...
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Enzyme Technology Technology used to preserve various forms of food such as fruit and vegetables can often rely heavily on natural resources such as the utilization of fossil fuels for energy consumption. Enzymes can be used as an alternative method to the pre-established technologies many of which are unsustainable in the food processing industry. Enzyme technology is an efficient method...
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Abstract Resources in Indonesia are very large, especially sources that are food. One of the abundant resources in Indonesia is its fisheries resources, therefore the food must be processed in such a way as to have good quality and quality. Good quality and quality can help increase economic value and nutritional value in the community. There are many ways that...
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Abstract The purpose of the investigation was to examine the correlation of enzyme production in response to the evolutionary impact of AMY1 gene copy numbers in individuals. Based upon research obtained in previous studies, there was an assumed positive correlation between gene copy numbers and the production of salivary amylase protein levels in mg/mL. Variances in copy numbers can be...
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Abstract Digestive enzymes play a very important role in providing the energy and proteins that the body needs to survive from the food that is ingested. One important digestive enzyme is pepsin. This enzyme survives the acidic pH of the stomach and is not denatured by it as some enzymes might. Pepsin has a precursor, pepsinogen, which is the inactive...
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Enzymes are proteins that help with digestion and metabolism. Their function is to speed up certain reactions within cells. In this case, enzymes were part of a reaction with iodine. A total of eight test tubes were used; four for bacterial amylase and the other four for fungal amylase. A spot place was used to see the reaction between the...
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Introduction Enzymes are proteins that act as catalysts which accelerate many biological chemical reactions (Funk and Wagnalls 2018), making them an important staple in linking metabolic networks (Chiu et al. 2006) as well as many biological processes. Thus, it is important to have a fundamental understanding of enzymes and their reactions with substrates, including measuring their activity based on the...
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Introduction Starch is a common polysaccharide, made up of glucose monomers (Serrano and Peralta, 2015). Pancreatic amylase is often the enzyme involved in the digestion of starch (Chegeni et al., 2018). Amylase is also an exoenzyme and facilitates the digestion of dietary carbohydrates (Nifty et al., 2019). Amylase causes conformational changes in starch, resulting in a reduced viscosity, and increased...
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With the rising importance of enzyme-assisted extraction of essential oil from plant and microbial sources, researchers are now interested in studying the effect of combination of enzyme-assisted extraction and other non-conventional extraction techniques on the extraction of essential oil. Non-conventional techniques such as three-phase partitioning, microwave-assisted extraction, ultrasound-assisted extraction, and supercritical fluid extraction (Gupta et al. 2012). The combination of...
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Currently, the ongoing COVID-19 epidemic has led to an unprecedented shortage of disinfectants, hand sanitizers and detergents, especially in the United States and Europe. Hand sanitizer is regarded as an auxiliary product of hand soap and liquid. Since no vaccines or antiviral drugs have been developed to prevent or treat the SARS-CoV-2 epidemic, the importance of using hand sanitizer for...
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Biology needs a metabolic reaction at all times. This is because a special type of catalyst-enzymes are widely present in the body. It can effectively reduce the activation energy of each molecule participating in a chemical reaction, enabling the organism to complete various chemical reactions quickly and efficiently. Plant photosynthesis, human digestion and absorption of food all occur and complete...
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In the past 20 years, various enzyme preparations have been used in meat processing. The common points of enzyme preparations are: strong professionalism and can be carried out under mild conditions; low cost and energy consumption in processing; significant changes in meat properties and meat quality improvement; high production efficiency; No harmful ingredients remain in the meat. The usage of...
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Application in dairy products Lactose is a disaccharide found in mammalian milk and has low sweetness and solubility. Dietary lactose can improve the body's absorption of Ca, P, Mg and other essential trace elements, but it cannot be directly absorbed in the small intestine, and must be hydrolyzed by the small intestine to be digested and absorbed by the body....
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With the rapid development of the dairy cattle breeding industry and the dairy industry, the output of raw milk and dairy products has increased significantly, the variety of dairy products has been greatly enriched, and the quality has also made a qualitative leap. High and new technologies such as gene technology, enzyme technology, microbial technology, high-pressure technology, and cold sterilization...
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Enzyme is a protein molecule that takes on a particular shape which enables them to speed up biochemical reactions within the organisms, therefore behaving as a catalyst. It can also be used in industrial and medical contexts. Bread making, cheese making and beer brewing all depend on the activity on enzymes, and enzymes can be inhibited if their environment is...
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Oceans provide 90 percent of living space on our planet Earth by volume and about 95 percent of this enormous area is still unseen by humans. (1) This huge area holds infinite opportunities with a massive diversity of organisms. The plants, animals and all the other microorganisms, even the tiniest ones that live in the salt water of the sea...
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Enzymes are key protein molecules in living systems. Once synthesized, they are not usually converted to other kinds of molecules, and so are the substances taken in as fuel for digestive and respiratory processes (such as sugar, fat, and molecular oxygen). This is because enzymes are catalysts, which means they can participate in chemical reactions without themselves being changed, just...
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RATIONALE Enzymes are proteins that act as catalysts within living cells. Catalysts increase the rate at which chemical reactions occur without being consumed or permanently altered themselves by lowering their activation energy (Christensen, 2018). Every enzyme has a region called active site, where enzymes are binds to a substrate to create another enzyme and carries a reaction. Enzyme is important...
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In science, enzymes are being used as bio-catalysts, meaning they accelerate chemical reactions by lowering the activation energy. Enzymes play a significant role in the lives of living organisms as they give aid in metabolic activities such as respiration and digestion, as well as help to maintain homeostasis. Apart from this, enzymes work as substances which break-down particles like protein,...
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Abstract The purpose of this experiment was to investigate the glucose concentration in popular drinks coke and Gatorade and to identify how a changing environment can impact the hydrolysis of sucrose. This was examined by a series of mutli- enzymatic reactions of glucose resulting in NADPH, measured by a spectrophotometer at 340nm. The results showed the glucose concentration of coke...
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Introduction In our society, using enzymes or microorganisms in food preparation has become widely known. As new technology has been developed, new application fields have been developed and pioneered. Microorganisms such as bacteria, yeast and the other enzymes are highly used for improving the taste and texture and eventually obtain enormous economic benefits to industries. With the advancement of technology,...
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Multi-enzyme feed is a type of biological feed. This kind of feed is rich in a variety of feed enzymes, which can eliminate the anti-nutritional components in feed ingredients to a certain extent or play a supplementary role in animal endogenous enzymes. Therefore, it is beneficial to improve the quality of the existing feed and increase the utilization rate of...
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Rationale The research question that was decided was formed from the claim 'Crispr can produce individuals who are free of genetic disease.' An article posted on the Morning Edition mentioned that a New York scientist conducted gene-editing experiments which, although raised some ethical concerns, could someday prevent many inherited diseases. Before the final question could be developed, numerous elements from...
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With nutrients being consumed daily by individuals, this lab serves to inform one of the processes of osmosis and diffusion when digested, as well as detecting the nutrient that will result in either process. Due to the human viewing the objective, they must be informed of the processes that occur within their bodies, hence the usage of a semi-permeable membrane...
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Abstract The experiment that was performed over two weeks served to examine how the amount of enzyme, amount of substrate, presence of inhibitor, or the magnitude of temperature changes the reaction rate between the enzyme and substrate present. The enzyme used in these experiments was peroxidase which was extracted from turnips and placed in flasks to be transferred into tubes...
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Abstract The purpose of this experiment was to determine the optimal temperature for both bacterial enzyme amylase, known as Bacillus licheniformis (B. licheniformis), and fungal enzyme amylase, known as Aspergillus oryzae (A. oryzae). During this experiment, both enzymes were exposed to 4 different temperatures (0 ᵒC, 25 ᵒC, 55 ᵒC, and 85 ᵒC) for specific time intervals. The time intervals...
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